Simultaneous method development and validation of 1, 8-Cineole and Betulinic acid in Shati Vati - An Ayurvedic formulation by HPTLC

A simple, accurate and precise HPTLC method was developed validated for simultaneous estimation of 1, 8-Cineole Betulinic acid in Shati Vati - An Ayurvedic formulation by HPTLC. contains rhizome powder have 1-8 cineole Badar ripe fruit reported medicinal claims uses. In the analytical method, toluene: ethyl acetate (93:07 v/v) finalized as mobile phase a clear separation achieved. The wavelength 665nm 580nm respectively. Rf value betulinic obtained 0.647 0.213 Linearity, Accuracy, Precision, LOD, LOQ Robustness per ICH Q2 (R1) guideline. Results validation process were within range acceptance criteria been applied successfully Cineole an ayurvedic formulation.